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cd4+cd25+ regulatory t cell isolation kit, mouse  (Miltenyi Biotec)


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    Miltenyi Biotec cd4+cd25+ regulatory t cell isolation kit, mouse
    Cd4+Cd25+ Regulatory T Cell Isolation Kit, Mouse, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 96/100, based on 232 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cd4+kit/CD4%2BCD25%2B+Regulatory+T+Cell+Isolation+Kit%2C+mouse/custom%40130-091-041%4042480527
    Average 96 stars, based on 232 article reviews
    cd4+cd25+ regulatory t cell isolation kit, mouse - by Bioz Stars, 2026-10
    96/100 stars

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    Related Articles

    Lysis:

    Article Title: Regulation of Tph cell differentiation via farnesoid X receptor of dendritic cells in inflammatory bowel disease
    Article Snippet: .. After erythrocyte lysis, cell pellets were resuspended and naïve CD4 + T cells were isolated using the Naïve CD4 + T Cell Isolation Kit (130-104-453, Miltenyi, Germany) according to the manufacturer’s instructions. .. For the BMDC–T cell co-culture system, 3 × 10 5 naïve CD4 + T cells from WT or Fxr −/− mice were activated with plate-bound anti-CD3e (16–0031-85, eBioscience, 5ug/ml) for 24 h. They were then co-cultured with 3 × 10 4 BMDCs from WT or Fxr −/− mice with or without IL-12 (10 ng/mL, PeproTech, USA) in 96-well round-bottom plates for 72 h (37°C, 5% CO 2 ).

    Isolation:

    Article Title: Regulation of Tph cell differentiation via farnesoid X receptor of dendritic cells in inflammatory bowel disease
    Article Snippet: .. After erythrocyte lysis, cell pellets were resuspended and naïve CD4 + T cells were isolated using the Naïve CD4 + T Cell Isolation Kit (130-104-453, Miltenyi, Germany) according to the manufacturer’s instructions. .. For the BMDC–T cell co-culture system, 3 × 10 5 naïve CD4 + T cells from WT or Fxr −/− mice were activated with plate-bound anti-CD3e (16–0031-85, eBioscience, 5ug/ml) for 24 h. They were then co-cultured with 3 × 10 4 BMDCs from WT or Fxr −/− mice with or without IL-12 (10 ng/mL, PeproTech, USA) in 96-well round-bottom plates for 72 h (37°C, 5% CO 2 ).

    Cell Isolation:

    Article Title: Regulation of Tph cell differentiation via farnesoid X receptor of dendritic cells in inflammatory bowel disease
    Article Snippet: .. After erythrocyte lysis, cell pellets were resuspended and naïve CD4 + T cells were isolated using the Naïve CD4 + T Cell Isolation Kit (130-104-453, Miltenyi, Germany) according to the manufacturer’s instructions. .. For the BMDC–T cell co-culture system, 3 × 10 5 naïve CD4 + T cells from WT or Fxr −/− mice were activated with plate-bound anti-CD3e (16–0031-85, eBioscience, 5ug/ml) for 24 h. They were then co-cultured with 3 × 10 4 BMDCs from WT or Fxr −/− mice with or without IL-12 (10 ng/mL, PeproTech, USA) in 96-well round-bottom plates for 72 h (37°C, 5% CO 2 ).



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    TRPM2 deficiency is associated with attenuated Ca 2+ ‐NFAT signaling during T cell activation. (A) Intracellular Ca 2+ flux in splenic <t>CD4</t> + T cells from WT and TRPM2 −/− mice, measured by flow cytometry using Fluo‐4 AM after stimulation with anti‐CD3/CD28 antibodies (5 μg/mL each). The graph shows the mean fluorescence intensity (MFI) over time from one representative experiment of three independent replicates. (B) Nuclear translocation of NFATc1 and IL‐2 production were assessed by Western blot from WT and TRPM2 −/− mice.
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    Image Search Results


    TRPM2 deficiency is associated with attenuated Ca 2+ ‐NFAT signaling during T cell activation. (A) Intracellular Ca 2+ flux in splenic CD4 + T cells from WT and TRPM2 −/− mice, measured by flow cytometry using Fluo‐4 AM after stimulation with anti‐CD3/CD28 antibodies (5 μg/mL each). The graph shows the mean fluorescence intensity (MFI) over time from one representative experiment of three independent replicates. (B) Nuclear translocation of NFATc1 and IL‐2 production were assessed by Western blot from WT and TRPM2 −/− mice.

    Journal: Immunity, Inflammation and Disease

    Article Title: TRPM2 Deficiency Attenuates Allergic Rhinitis‐Like Inflammation With Altered Ca 2+ ‐NFAT Signaling, Treg Responses, and sIgE Production

    doi: 10.1002/iid3.70490

    Figure Lengend Snippet: TRPM2 deficiency is associated with attenuated Ca 2+ ‐NFAT signaling during T cell activation. (A) Intracellular Ca 2+ flux in splenic CD4 + T cells from WT and TRPM2 −/− mice, measured by flow cytometry using Fluo‐4 AM after stimulation with anti‐CD3/CD28 antibodies (5 μg/mL each). The graph shows the mean fluorescence intensity (MFI) over time from one representative experiment of three independent replicates. (B) Nuclear translocation of NFATc1 and IL‐2 production were assessed by Western blot from WT and TRPM2 −/− mice.

    Article Snippet: Naïve CD4 + T cells were then isolated by negative selection using a commercial mouse CD4 + T Cell Isolation Kit (Cat. No. 130‐104‐454, Miltenyi Biotec, Bergisch Gladbach, Germany), according to the manufacturer's instructions.

    Techniques: Activation Assay, Flow Cytometry, Fluorescence, Translocation Assay, Western Blot